Purpose: This study aimed to identify the effects of pelvic floor dysfunction, life stress, and social support on quality of life in middle-aged women. Methods: A total of 150 middle-aged women aged 40–60 years who understood the purpose of the study and voluntarily agreed to participate were included. Data were collected from October 20 to October 31, 2025, through an online survey distributed via announcements posted at community centers, apartments, and online communities in Changwon. Data were analyzed using SPSS WIN 23.0, including descriptive statistics, t-tests, one-way ANOVA, Scheffé’s tests, Pearson correlation coefficients, and multiple regression analyses. Results: Multiple regression analysis revealed that significant predictors of quality of life were perceived health status (β=−.37, p<.001), social support (β=.34, p<.001), life stress (β=−.22, p=.016), and economic status (β=.15, p=.043). Higher social support, higher perceived health, lower life stress, and moderate economic status were associated with a higher quality of life. Conclusion: The findings indicate that enhancing social support, improving health status, reducing life stress, and promoting economic stability are essential factors for improving quality of life among middle-aged women. These results highlight the need for targeted interventions and supportive policies to address these key determinants of a better quality of life.