Purpose: The purpose of this study was to investigate the e-health literacy, internet health information reliability, and health promotion behavior of university students, and to provide basic data for developing a program to improve health promotion behavior of university students. Methods: The subjects of this study were 148 students, and data were collected through an online surveys from April 7 to April 14, 2023. The collected data were analyzed using the SPSS program for descriptive statistics, t-test, ANOVA, Pearson’s correlation, and regression analysis. Results: As a result, students’ e-health literacy was 3.78±0.59, Internet health information reliability was 3.56±0.59, and health promotion behavior was 2.62±0.51. The health promotion behavior of students was statistically correlated with Internet health information reliability (r=.45, p<.001) and e-health literacy (r=.33, p<.001). The variables that significantly affected students’ health promotion behaviors were Internet health information reliability (β=.38, p<.001), subjective health status (β=.19, p=.015), and health concern (β=.18, p=.015), and the explanatory power of these variables was 26.5%. Conclusion: Based on the results of this study, it is necessary to develop a program to improve Internet health information reliability, individual health status awareness, and health concern in order to improve university students’ health promotion behavior.