Purpose: This study aimed to identify factors associated with breast self-examination (BSE) practice among Korean women. Methods: A descriptive cross-sectional survey was conducted using an online self-administered questionnaire from November to December 2020. Eighty-three Korean women aged 20–69 years were included in the final analysis. The questionnaire assessed general and BSE-related characteristics, BSE knowledge, health beliefs regarding BSE, and BSE practice. BSE practice was dichotomized into practice and non-practice groups. Data were analyzed using descriptive statistics and exploratory logistic regression analysis with IBM SPSS Statistics version 25.0. Results: Among the participants, 85.5% were aware of BSE, whereas only 6.0% reported practicing BSE regularly. Logistic regression analysis revealed that family history of breast cancer (odds ratio [OR]=0.20, 95% confidence interval [CI]=0.04–0.91, p=.047), knowledge of how to perform BSE (OR=0.10, 95% CI=0.03–0.38, p=.001), and confidence in performing BSE (OR=0.92, 95% CI=0.86–0.98, p=.015) were significantly associated with BSE practice. The final model was statistically significant (χ²=23.13, p<.001), demonstrated adequate goodness of fit, and explained 32.5% of the variance in BSE practice. Conclusion: The results suggest that BSE practice among Korean women is more closely related to practical method knowledge and confidence than to general knowledge alone. Standardized community-based BSE education programs should emphasize hands-on practice, confidence building, and sustained breast awareness